Archive of the blog

Apr 2, 2014

Genetic diversity and structure ofJatropha curcas L. in its centre of origin

M. Salvador-Figueroa, J. Magana-Ramos, J.A. Vazquez-Ovando, M.L. Adriano-Anaya and I. Ovando-Medina

To investigate the genetic diversity and structure of Jatropha curcas L. oilseed plant, in this study, native populations from Chiapas, Mexico, were evaluated, using microsatellite DNA markers. A total of 93 representative samples were selected from seven sites in two regions in the state of Chiapas grouped by geographical proximity, where leaf samples were collected to isolate the genomic DNA. Individual polymerase chain reactions were carried out with ten pairs of specific oligonucleotides for the microsatellites of J. curcas, separating the products of amplification by acrylamide electrophoresis. Twenty-seven fragments were detected (77% polymorphic) with which heterozygous individuals were distinguished. The most informative microsatellite was Jcps20 (nine alleles, polymorphic index content 0.354). The average poly- morphism per population was 58%. The Hardy–Weinberg tests revealed a reproductive pattern of non-random mating. The diversity descriptors and the analysis of molecular variance revealed that the populations were structured and moderately differentiated (FST 0.087) and that this differentiation was not due to isolation by distance, as the Mantel test was not signifi- cant (P 1⁄4 0.137), but rather due to allopatry. Bayesian analysis revealed that the accessions belonged to only four genetic groups and confirmed the differentiation between the regions. Because some loci were in Hardy–Weinberg disequilibrium, it is proposed that differentiation is due to the clonal reproduction of J. curcas practised by farmers in Chiapas, along with the anthropogenic dispersion at regional levels. The results of this study reveal that J. curcas in Chiapas has genetic diversity that is greater than that reported in other parts of the world, which represents a potential germplasm pool for the selection of genotypes. 

Feb 27, 2014

Genetic diversity of Jatropha curcas in Southern Mexico

Authors: Isidro Ovando-Medina, Lourdes Adriano-Anaya, Alfredo Vázquez-Ovando, Sonia Ruiz-González, Manuel Rincón-Rabanales, Miguel Salvador-Figueroa
Publication date: 2013/1/1 
Book title: Jatropha, challenges for a new energy crop
The importance of the Euphorbiaceous plant Jatropha curcas L. lies in its high-quality seed oil, ideal for the manufacture of biodiesel. As a result, its extensive cultivation has already reached several million hectares in Asia, Africa and Latin America, and implies certain challenges since it is not yet a fully domesticated plant, and with limited information  on the agronomic processes and selected varieties for its cultivation. In addition, little is known about the biology, ecology, genetic diversity and geographic origin of its ...

Biofertilization of micropropagated Agave tequilana: Effect on plant growth and production of hydrolytic enzymes

Authors: Sonia Ruiz, Lourdes Adriano, Isidro Ovando, Cuauhtemoc Navarro, Miguel Salvador

Publication date: 2013/10/24

Journal name: African Journal of Biotechnology

 Abstract Three beneficial bacterial strains [Gluconoacetobacter diazotrophicus (Pal5), the 
diazotrophs (11B) and Pachaz (008)] and an arbuscular mycorrhizal fungus [Glomus 
intraradices (AMF)] were evaluated for their ability to enhance plant growth and the 
production of hydrolytic enzymes in micropropagated Agave tequilana Weber var. Blue. 
Results show that the growth of the agave plants and the production of hydrolytic enzymes in 
their roots were influenced by the presence of these microorganisms. AMF+ 11B treatment ...

Genetic Diversity in Jatropha curcas Populations in the State of Chiapas, Mexico.

Authors: Isidro Ovando-Medina, Adriana Sánchez-Gutiérrez, Lourdes Adriano-Anaya, Francisco Espinosa-García, Juan Núñez-Farfán, Miguel Salvador-Figueroa 

Publication date2011/12/1

Journal name: Diversity (14242818

Abstract Jatropha curcas L. has become an important source of oil production for biodiesel 
fuel. Most genetic studies of this plant have been conducted with Asian and African accessions, where low diversity was encountered. There are no studies of this kind focusing in the postulated region of origin. Therefore, five populations of J. curcas were studied in the state of Chiapas, Mexico, using amplified fragment length polymorphism (AFLP) markers. One hundred and fifty-two useful markers were obtained: overall polymorphism= 81.18% ...

Authors:  Miguel Salvador-Figueroa, Emilio Hernández-Ortiz, Carmen Ventura-González, Isidro Ovando-Medina, Lourdes Adriano-Anaya

Publication date: 2013

Journal name: Revista Iberoamericana de Tecnología Postcosecha

We investigate the effect of chitosan coatings to kill the immature stages of Anastrepha ludens
(Mexican fruit fly) in mango fruits (cv. Ataulfo). The fruits were effectively infested after exposure of 4 h to gravid females. Larvae of first, second and third instars were found inside the fruits 3, 7 and 10 d after oviposition. No embryos, first or second larval instar was developed when the fruits were coated 0.5, 2, 4 and 8 d after oviposition. We suggest the mass transfer (CO2 and O2) modification can be implicated in those results. In contrast, application of chitosan ...

Aug 27, 2012




Mar 24, 2011

State of the Art of Genetic Diversity Research in Jatropha curcas

I. Ovando-Medina, F. Espinosa, J. Núñez-Farfán & M. Salvador. 

Scientific Research and Essays. In correction.

Evolution of the investigations on Jatropha curcas genetic diversity, published in indexed journals the last fiftheen years, by region of origin of the germplasm analyzed.

Dec 14, 2010

Artículos sobre genética de Jatropha curcas mesoamericana

Genetic Variation in Mexican Jatropha curcas L. Estimated with Seed Oil Fatty Acids 
I.Ovando-Medina, F. Espinosa-García, J. Núñez-Farfán and Miguel Salvador-Figueroa
Journal of Oleo Science 60 (No. 7, 2011) In press.

Map showing the sites of collection of Jatropha curcas evaluated in their composition of oil from Southern Mexico and from Guatemala, and the two main genetic barriers (lines “a” and “b” in yellow) found by the algorithm of Monmonier (Barrier vers. 2.2), as based on Fisher distances of fatty acid composition of the seed.
Read it in PDF format in:

Nov 25, 2010

Read my papers (full text)

Does Biodiesel from Jatropha CurcasRepresent a Sustainable Alternative Energy Source?

Isidro Ovando-Medina, Francisco Espinosa-García, Juan

Núñez-Farfán and Miguel Salvador-Figueroa.


Various government agencies around the world have proposed vegetable oils and their conversion to biodiesel as a renewable alternative to fossil fuels. Due to its adaptability to marginal soils and environments, the cultivation ofJatropha curcas is frequently mentioned as the best option for producing biodiesel. In the present work the current situation of proven and potential reserves of fossil fuel, and the production and consumption model for the same are analyzed, in order to later review the sustainability of the production process which begins with the cultivation of J. curcas, and culminates with the consumption of biodiesel. A review of the following topics is proposed in order to improve the sustainability of the process: areas destined for cultivation, use of external (chemical) inputs in cultivation, processes for converting the vegetable oil to biodiesel, and, above all, the location for ultimate consumption of the biofuel.

Download in PDF from:

Influencia de la inoculación de diazótrofos y del tipo de suelo en la germinación y crecimiento inicial de
Jatropha curcas L.

Ingrid Donají, Lourdes Adriano, Isidro Ovando y Miguel Salvador.

Download in PDF from:

Ex vitro survival and early growth of Alpinia purpurata plantlets inoculated with Azotobacter and Azospirillum.

Ovando-Medina I, Adriano-Anaya L, Chávez-Aguilar A, Oliva-Llaven A, Ayora-Talavera T, Dendooven L, Gutiérrez-Miceli F, Salvador-Figueroa M.


The survival rate, shoot and root dry mass, shout number, plant growth, stem height and diameter, number of leaves and root length were measured in micropropagated plantlets of Alpinia purpurata (Red ginger) inoculated with Azospirillum sp. 11B and Azotobacter sp. Pachaz 008 at 10(7), 10(8) and 10(9) cells cm(-3) using a complete randomized experimental design. Inoculation ofA. purpurata plantlets with the Azospirillum sp. 11B or Azotobacter sp. PACHAZ 008 strains induced larger stem diameter, root dry mass, number of shoots and increased their survival rate from 77 to 100% compared to plantlets without inoculation, while other plant characteristics were not affected.

Download in PDF from:

Micropropagación de plantas ornamentales tropicales en medios de cultivo basados en "humus líquido" de vermicomposta.

Gemelli López Martínez, Isidro Ovando Medina, Lourdes Adriano A., Raúl Cuevas González y Miguel Salvador F.

Download in PDF from:

Current List of the Native Bromeliads of Soconusco, Chiapas, Southeast Mexico.

Dayam Santiago , Sonia Ruiz , Lourdes Adriano , Miguel Salvador and Isidro Ovando-Medina.


In Central America, native bromeliads are an alternative for productive diversification in coffee plantations; however, first, a census in each area should be carried out and inventories must be up-dated. During 2004 and 2005, field explorations were carried out in the Soconusco region, on the Mexico/Guatemala border, in order to collect live specimens and to create a reference collection of plants in the University (UNACH, Universidad Autónoma de Chiapas, Tapachula). To verify if the collected species were native or not, an ethnobotanical study was carried out, with semi-structured interviews applied to people from the visited communities; the information was then compared with the literature. The study revealed few uses of native bromeliads. To date, we have 186 accessions which had been characterized and classified in 6 genera and 29 species. This number of species comprises only 22.8% of the bromeliads of Chiapas, but represents a high density of species (3.113 species/1000 km2). Most of the accessions are from middle altitudes (500-1500 m.a.s.L.). We present the first inventory of the flora Bromeliaceae for the region of Soconusco.

Download in PDF from: